Absorbance was then determined at 450 nm by SpectraMax Paradigm microplate reader (Molecular. The reader adds up to three transfers of assay reagents from a 96- or 384-well source plate to enhance assay design using integrated fluidics. A Costar® 24-well tissue culture plate with col1 = blanks and doubling cell concentration left to right starting at Col. A = 2 - log 10 (%T). Explore the best practices for running absorbance-based assays how Molecular Devices absorbance readers can be utilized to optimize said absorbance-based assays. Pick the best speed, sensitivity, and price for your needs. It is designed to work seamlessly with a variety of 96 well microplate readers, including popular brands such as Agilent™, Berthold™, Biotek™, BMG LABTECH™, Molecular Devices™, Perkin Elmer™, Promega™, Tecan™, and. Devices® SPECTRAmax 190 microplate reader at a wavelength of 600 nm. 012 fmol 0. 00 . 1. The DynaPro Plate Reader III provides unparalleled levels of productivity and flexibility for the most demanding nanoparticle sizing and protein aggregation tasks such as biotherapeutic formulation, screening of promiscuous inhibitors, and optimization of protein. 003 fmol 0. These include real-time PCR, measuring yields of RNA preparations for microarray experiments, reverse transcription PCR (RT-PCR), differential display PCR,. 500 OD ± 3. These robust readers have been placed in labs from Antarctica to. The SpectraMax ABS/ABS Plus Microplate Readers provide fast absorbance detection without the use of filters with monochromator-based wavelength selection for visible and UV-visible absorbance. #6590-0137) Optimal instrument settings for ValitaTiter (P/N VAL003 or VAL013, left table) and ValitaTiter Plus (P/N VAL004 or VAL014, right table) assays on the SpectraMax iD5 reader are shown below. The BCA assay plate was read at 562 nm, the original wavelength setting in the protocol, while for the BCA Rapid. The system features 96, 384,. Quickly image a variety of plate formats and cell types - Image a 96-well plate in under two minutes. *For Corning UV plates, mean values at 260 nm ranged from 0. Instrument design, user interface capabilities, and cost play key roles in purchasing a suitable microplate reader. None for any other plate (6, 12, 24, 48 well plates) Flex3: Both Top read and. SpectraMax iD5 Multi-Mode Microplate Reader (Molecular Devices cat. SpectraMax L Microplate Reader is a sensitive luminometer with programmable injector options that measures flash and glow luminescence assays, including dual luciferase. Uncontrolled Copy for Training and Reference Purposes Only . Download a PDF version of the SpectraTest validation package User Guide and the ABS2 SpectraTest validation protocols. Quantitative, whole well cell confluence for every well. Some instruments can handle only 96-well plates and lower, whereas others can read up to the ultrahigh throughput densities of 1,536-well plates. Before dispensing reagents to the microplate, the VersaMax Reader was powered on and. SpectraMax L Microplate Reader is a sensitive luminometer with programmable injector options that measures flash and glow luminescence assays, including dual luciferase reporter gene, G protein-coupled receptor (GPCR) in 96- and 384-well plates. Evaporation protection (humidity cassette) Te-Cool™ (active temperature regulation 18-42 °C) Integrated lid handling. Three low volume 24-well bottom slides. High sensitivity and speed are required for these plate formats if you wish to examine quick kinetic measurements such as calcium fluxes or applications with small signal. The VMax Microplate Reader is capable of reading up to two wavelengths at a time in the visible wavelength spectrum (400nm-750nm), with 4 filter choices included at time of purchase. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a variety of assays. 0001 ABS (calculated) • Light source: Halogen lamp, 8V/50W • Wavelength range: 405, 450, 492, 630 nm, 4 more filters optional. The trademarks used herein are the property of Molecular Devices, LLC or their respective owners. Microplate spectrophotometers, also called microplate readers, allow researchers to analyze multiple samples in parallel using 6- to 1536-well formats, as opposed to single sample measurements. The variations include adjustable speed and volume, adjustable aspiration speed and time, adjustable soak times, and three modes of shaking. Sensitive luminometer with programmable injector options for 96- and 384-well microplates. To test the optical clarity of the various microplates at low wavelengths, one well of each plate was filled with 100 μL (96-well) or 20 μL (384-well) distilled water, and its. To assess the sensitivity of each microplate reader, a plate was set up with 32 wells containing PBS only. Contact our support team with your questions regarding Molecular Devices products and applications. µl) was added to each well and incubated at 37°C for 1 h. LogPhase 600 is controlled with a software App that provides a simple, yet powerful, user experience. 40266. The trademarks used herein are the property of Molecular Devices, LLC or their respective owners. Other technologies such as imaging. with Quartz ABS Plastic. 96-well) while others have flexibility to. Microplate Spectrophotometer Operator's Manual Molecular Devices Corporation 1311 Orleans Drive Sunnyvale, California 94089 Part # 0112-0126 Rev A. 1 OD at 230 nm. Top read: purple adapter (p/n: 0310-4336) Bottom read: black adapter (p/n: 0310-4089) Flex mode always uses black adapter because it's always bottom read. Temperature variation ± 0. Transfer the assay plate directly to a bottom-read fluorescence microplate reader for kinetic read-mode or incubate for 10-30 min at 37oC and then read for endpoint read-mode. Detect dim samples with unsurpassed sensitivity; Avoid saturation issues with nine orders of dynamic range. XTR Rack Pro 96 (Vial Rack Bottom Label 2D Reader) BIO-LAYER INTERFEROMETRY MICROPLATE READING: Forte Bio: Octet HTX: Octet QK384:. SpectraTest FL1 Fluorescence Validation Plate User Guide Molecular DevicesData are shown as the mean ± s. Shaking: Linear, orbital, and double orbital. 190. 100 fmol luciferase per well. the Molecular Devices SPECTRAmax® 190 and 250. Polypropylene Deep well Plates are used as small test tubes because of easier sealing, flat or conical shape, square and round well formats with lids and cap. 5 pM Luminescence performance Wavelength range 300–850 nm Wavelength selection Choice of simultaneous detection of all wavelengths or selection in 1. Step 1: Capture antibody binds to ELISA plate wells. Prior to the reading of each plate, the microplate reader was “blanked” with a plate containing 0. #R8340) SpectraMax i3x Multi-Mode Microplate Reader (Molecular Devices cat. Plate. It offers extremely low absorbance background, excellent well to well uniformity, and zero percent cross talk. absorbance microplate reader GEA. With the novel filter system, assays such as hydrogen peroxide detection assays and IRantibody-binding assays can be run confidently with Molecular Devices. CO 2 & O 2 control. Running both standard spectrophotometer and microplate. Microplate readers from Tecan; cutting edge bench-top microplate readers lead the industry in both versatility and performance. Page 38 SpectraMax iD3 Multi-Mode Microplate Reader User Guide Bottle Holder The bottle holder is mounted on two knobs for ease of installation, cleaning, and replacement. No. Categories: Plate Readers. # SMP54-GXP- 10UL) Pyrochrome Assay chromogenic test reagent (ACC cat. SpectraMax 340PC384 Microplate Reader A versatile 96- and 384-well format absorbance reader let the monochromator tune to that exact wavelength. Well-to-WellPrecision RFULinearity RelativeFluorescenceUnit(RFU)ScaleRatio(GeminiEM,XPS,SpectraMaxM2-M5e, FlexStation3only). Microplate Capacity. The four standard filters – 405, 450, 492, 620 nm – allow for a wide variety of ELISAs such as PNPP, ABTS, OPD and TMB. This chapter reviews key criteria for microplate selection, including: well number, well volume and shape,. (96- or 384-well) simultaneously and with high temporal resolution. Narrow bandwidths deliver increased measurement accuracy and linearity over the widest range of assays, including. The EMax® Endpoint ELISA Microplate Reader from Molecular Devices, one of the pioneers in microplate detection, offers a ruggedly simple approach to dedicated endpoint assays. 0 . Fluorescence, Absorbance Fastest Read Time - 96-well plate: 20 sec; 384-well plate: 30 sec Temperature Control: Ambient +5C up to 42C Shaking: Linear and Orbital. B. Microplate washers are designed for effortless use with 96- or 384-well plates in routine ELISA applications. Related products. Molecular Devices: FlexStation 3: Multimodal: Limited - monochronometer based: N/A:the iD5 reader’s filter system. Optional 16-way manifold. Clear plates will give more cross talk between wells. An example of dual-luciferase assay results. maximum-illumination-channels. Ensure that cells reach around 80–90% of confluence on the day of the. UV-Star ® 96W Microplate, well working volume 25-370 μL; total well volume 392 μL. A custom 96-well plastic (polyethylene terephthalate/PET) plate is attached via epoxy to the mounted CMOS ICs, designed with a standard form factor to be compatible with high-throughput. Detection modalities include absorbance (UV-Vis Abs), fluorescence intensity (FI), luminescence (Lumi), and all have. Gemini XPS & EM readers measure fluorescence on a variety of sample formats from 6- to 384-well microplates. luminescence for a 96-well plate and 40 msec integration time for a 384-well plate: 0517 Lit. Compatible with Molecular Devices SPECTRAmax 190 and 250 plate readers. Through absorbance readings from both the ultraviolet and visible spectrum range, it can determine the concentration of samples, conduct positive or negative detection of antibodies and. Select up to 3 products. External Dimensions without lid (mm). Only Molecular Devices microplate plate readers offer the capability to measure the depth (optical pathlength) of samples with no temperature dependency using the patented PathCheck® Sensor technology. Top and bottom reads are. 2. For a higher degree of automation, the Automation Vendor Partners Program has streamlined the integration of our microplate reader systems with all leading partner. of 3 wells of a 96-well plate, from 1 of 3 independent experiments. EMax. This is equivalent to having 511 built-in filters, The instruments offer 96- and 384-well microplate-to-microplate fluid transfer (8 or 16 wells at a time), and can read microplates having 6, 12, 24, 48, 96, and 384-well formats. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the. 4 decades . Polypropylene deep well plates, round bottom. SpectraMax and VersaMax Plate Readers OperatorÕs Manual Ñ 0112-0126 Rev A SpectraMax Plus 384. Capabilities. Unparalleled performance on a personalized platform. $488. The plate reader optics are tunable for absorbance, fluorescence intensity, fluorescence polarization, and time resolved fluorescence assays (TRF). Range of 96, 384-well SBS microplates . The EMax Plus Microplate Reader measures 96-well plates and comes with eight standard filter modes to cover the entire visible range, for applications such as protein quantification, cell viability, cell proliferation and ELISA. $3,450. Luminescence applications and assays. Absorbance Linearity: 2. as well as all other Molecular Devices' microplate readers. Our. The VMax Microplate Reader is capable of reading up to two wavelengths at a time in the visible wavelength spectrum (400nm-750nm) with 4 filter choices included at time of purchase. No additional manuals are available. Description. Reconstitution of. Spectrofluorometers or plate readers with fluorescence detection typically offer greater sensitivity and a wider dynamic range compared to absorbance detection. x. Another consideration, says Amouretti, is plate format. Turn on Plate Reader and wait for it to complete its start-up routine. The Versamax Microplate Reader is built around a grating monochromator. Microplate format. Well scans from the Gemini XPS Reader illustrating serial dilution of CHO cells using the Live Cell assay from Molecular Probes Live/Dead (Calcein AM /Ethidium Homodimer-1) assay kit. For more sample throughput, the system can be easily integrated into a full robotic The SpectraMax® Paradigm® Multi-Mode Microplate Reader measures absorbance, fluorescence, time-resolved fluorescence (including HTRF), fluorescence polarization, AlphaScreen®, AlphaLISA®, and luminescence assays for up to 1536-well plates. The onboard luminescence detection mode may be used, or, for higher sensitivity, the reader can be equipped with a NanoBRET detection cartridge that offers a 4-fold improvement in limit of quantitation. Fireflysci UV/VIS Fluorescence Validation Plate for Well Plate Reader (290-562nm) FLVP-01. Making it possible to perform kinetic analysis of solid. Click Create. flat-bottom: 50-60 µl. This study investigated the efficacy of fermented seaweed (Eucheuma cottonii) on the remediation of fluoride-contaminated soil. SpectraDrop High-Throughput Screening (HTS) Kit. DynaPro ™ Plate Reader III. x. An entire 96-well plate can be processed in less than six seconds. Absorbance Plate Reader Comparison Chart. The absorbance was then read on the SpectraMax microplate reader at 426 nm, and the resulting OD values for all wells were averaged together to obtain a value of 0. Flexible liquid transfer Using an 8- or 16-channel pipettor, the FlexStation 3 Reader offers added assay flexibility over dispenser-based systems by transferring reagents from 96 or 384 distinct wells in a source plate to a read plate, simultaneously. 100 ˜L (96-well) or 25 ˜L (384-well) of Loading Buffer was added to each assay well. Find our technical support information along with contact details. For the name of the nearest authorized Molecular Devices Corporation service facility please contact Molecular Devices at one of the following telephone numbers: (408) 747-1700 (800) 635-5577 (U. Figure 2-23: Bottle holder To install the bottle holder: 1. 0 nm increments Dynamic range > 7 decades Cross-talk < 0. 2 pM 384–well: 3 pM 96–well: 2 pM 384–well: 4 pM 1 0200-7012POS Glow for 96-, 384-, and 1536-well plates Here, we demonstrate that with Molecular Devices SpectraMax® microplate readers and the Quant-iT PicoGreen assay, users can reliably measure concentrations as low as 50 pg/mL of double-stranded DNA. SpectraMax and VersaMax Plate Readers Operator’s Manual — 0112-0126 Rev A SpectraMax Plus 384. This is equivalent to having 511 built-in filters,The instruments offer 96- and 384-well microplate-to-microplate fluid transfer (8 or 16 wells at a time), and can read microplates having 6, 12, 24, 48, 96, and 384-well formats. The choice of microplate is a crucial yet often overlooked technical decision in HTS and quantitative biology. Infinite possibilities. Powerful, benchtop microplate stacker that works seamlessly with Molecular Devices microplate readers and washers. Microplate optimization and read height optimization were performed for best results. This 96-well assay has been fully validated on the SpectraMax iD5 reader and other Molecular Devices microplate readers with FP detection to ensure reliable results. 96-well plates - Filter plates with 0. 96-well Molecular Devices P/N 42-000-0117 HE™ High Efficiency microplates, 384-well Molecular Devices P/N 0200-5201. 0 nm increments Dynamic range > 7 decades Cross-talk < 0. #CA961) Methods. Sample by eye within . The SpectraMax Quant dsDNA Assay Kits provide pre-optimized, complete solutions that are sensitive, accurate, and easy to use. Read the microplate • Make sure the purple plate adapter (if required for your plate reader) is in the microplate reader drawer. • High antibody-binding surface improves the overall performance in. 993 (Figure 3), validating the assay’s usefulness over a wide range of viable cell numbers. Controlled by the computer. Kinetic readings were started immediately with a Molecular Devices FlexStation Plate Reader. Validation made easy -1) Select the correct plate and the wells that you want to measure. Type:Single Mode Absorbance Plate Formats:96 well plates Accuracy:0. It also integrates with the. Order Now. 001 ABS (displayed), 0. Molecular Devices SpectraMax Plus Microplate Reader Molecular Devices SpectraMax M3 Multi-Mode Microplate Reader Sale Price: $6,495. 100 μL of working reagent was added to each well after the 10-minute preincubation step. Microplates, cuvettes (via adapter) Detectors. VIS absorbance detection. Silica excitation fiber, glass emission fiber. 000 ABS • Resolution: 0. The optical system of the SpectraMax 190 Microplate Reader is built around a monochromator, which allows for selection of up to six wavelengths at a time for absorbance detection in the UV-visible wavelength range (190 nm - 850 nm). Transfer the assay plate directly to a bottom-read fluorescence microplate reader for kinetic read-mode or incubate for 10-30 min at 37oC and then read for endpoint read-mode. In labs where throughput is a top priority, a device that can screen 96- to 1536-well microplates in under one minute is worthwhile. 020 fmol 0. When the user applies a set temperature of 37°C, the top is heated to 37. Create custom fluorescence assays without having to purchase expensive filter sets. Integrated stacker option. Access this by going to. Here, we demonstrate validation of the SpectraMax ® i3x Multi-Mode Microplate Reader for NanoBRET assays. The. Microplate Spectrophotometers. The SpectraMax ABS readers can be integrated with Molecular Devices StakMax® Microplate Stacker within . The variations include adjustable. softmax pro software, gemini em reader, molecular devices stakmax microplate stacker, softmax pro data analysis, spectrofluorometer, monochromator microplate reader, cell migration assays, cell. Look for a washer which can handle a variety of plate types, including deep well plates and 96-, 384- and 1536-well plates. Usage/Application: Lab. 5 fg per well to 0. 3) At the bottom of the strip, click on "Show. Description: The SpectraMax® iD5 Multi-Mode Microplate Reader is the complete laboratory solution to help you increase your research. SpectraDrop Micro-Volume Refills, 24-Well Bottom Slides. is required for optimum performa nce with standard 96-well and 384-well format microplates for all top-read applications. MCF7 cells treated with. Site. Eight filters enable visible-wavelength absorption measurement applications such as protein quantification, cell viability and ELISA. Temperature control: 4°C above ambient to 45°C. (Molecular Devices cat. Molecular Devices has provided scientists with . The Molecular Devices SpectraMax 384 Plus is a UV/Vis spectrophotometer and microplate reader combination for more reliable and fast data reporting with built-in cuvette port and microplate drawer. These instruments deliver high precision and speed when reading 96 Well Microplates for single endpoint measurements and kinetic assay data gathered over time. When you do a read at wavelengths below 340 nm, you must use special UV-transparent, disposable or quartz plates to allow transmission of the deep UV spectra. The screen on the left shows the wells selected for Method MI AF488-10 and Method MI AF488-5 for image acquisition from rows A-D of a 96-well plate at. 00. Step 4: Add detection antibody – Enzyme-conjugated detection antibody binds to a second site on the. Molecular Devices (UK) Ltd. The ultimate guide to microplate readers is an eBook that tells you everything you need to know about plate reader solutions from what a microplate reader is, to popular applications and technology, to GxP compliance and special tips and tricks to help maximize your. They are widely used in research, drug discovery, [1] bioassay validation, quality control and manufacturing processes in the pharmaceutical and. Plates were read in the SpectraMax M5 microplate reader at 485ex/515em with bottom read. The plate reader optics are tunable for. When a. Fluorescence Microplate Reader Comparison Chart. Instrument pricing. A preconfigured protocol with optimized reader settings enabled reading of a 384-well plate in just two minutes. The plate was incubated for 10 minutes at room temperature and read at 595 nm on the SpectraMax ABS Plus reader. 2 with 1000 cells. x. ©2022 Molecular Devices - All rights. , Corning, NY) or Greiner. What plate adapters do you recommend for 96, 384-well plates for FlexStation? Flex/Flex II/384: Top read: purple adapter (p/n: 0310-4336) Bottom read: black adapter (p/n: 0310-4089) Flex mode always uses black adapter because it's always bottom read. Microplate readers, SpectraMax®, with SpectraDrop. 00. Read up to 2 wavelengths in endpoint absorbance for 96-well plates. • Gently wash cells once with 200 μl (for 96 well plate) or 400 μl (for a 24 well plate) warm (37°C) 1X DPBS (containing Mg and CaCl 2). These microplates have a corner notch for use in standard plate readers. Conventional technique: subjective, time-consuming. The SpectraMax® L microplate reader measures flash and glow luminescence assays in both 96- and 384-well plates. Wavelength(s):. The. 0% ± 0. x. The system can perform readings. For a higher degree of automation, the Automation Vendor Partners Program has streamlined the integration of our microplate reader systems with all leading partner robots. The SpectraMax® iD3 Multi-Mode Microplate Reader is the cornerstone of a complete laboratory solution designed to expand the boundaries of research capabilities. 057, depending on lot number. Figure 2. x. * Note: Programming the washer with a final aspirate height of 4. 96- and 384-well. Microplate readers read biological, chemical or physical events that occur on a microtiter plate. adjust the reader. SpectraMax M2 & SpectraMax M2e Multi-mode Plate Readers User Guide — 0112-0102 Rev. Molecular Devices의 Microplate Reader 및 washer와 원활하게 호환되는 강력한 벤치탑 Microplate stacker입니다. The instrument reads any standard cuvette, microcuvette, test tube or 96- and 384-well standard or UV transparent microplate over a full 190 to 1000. Luminometers and fluorimeters allow for readout of luminescence (luciferase readouts) or fluorescence from an entire well of a micro-well plate such as a 96- or 384-well plate. The 8-channel read head allows for fast read times which means a 96-well plate can be read in as little as five seconds. Time-resolved phosphorescence. the absorbance at 340 nm at 37 °C was measured every 30 s using SpectraMax M5 Microplate. Microplate Readers. Instrument settings can beCorning 96-well black microplates with clear bottom (Corning cat. • 0. Working in. 1. 150 µL of deionized water was pipetted into each well of a clean 96-well plate. Can read 96 well plates (optional 384 well). Both 96- and 384-well assay formats yielded similar linearity and dynamic range, indicating the suitability of the dual-luciferase assay and SpectraMax i3x system for both formats. Plate Shaking. Includes PC and software. The ImageXpress® Pico Automated Cell Imaging System is more than a digital microscope, combining high-resolution imaging with powerful analysis. 4ti-0255 96 well assay plate, pyramid flat bottom, clear, non-sterile UV wavelength 4ti-0214 UltraVision Plate 384, black, non -sterile, with UV baseELISA Workflow Using the EMax plus Microplate Reader and MultiWash+ Microplate Washer. After incubation of the cells with compounds, add 100 µL (for 96-well plates) or 25 µL (for 384-well plates) of Dye Solution per test well. 0 OD. Absorbance equation. The drawing shows a standard microplate of 15 mm height covered with a lid. Resipher 32x Starter Kit for use with 96-well plates Includes: (1) Resipher Data Hub, (1) Resipher 32x Reader, (3) sterile sensing lids, all cables Turns a 96-well plate into a precise dynamic oxygen consumption reader, measures 32 wells Utilizes Lucid Lab Cloud software for connection to any…. FlexIII. To maximize sensitivity of the assay, it is necessary to use optimal excitation and emission wavelengths. 340 PC384. The FlexStation® 3 Multi-Mode Microplate Reader can be used to run the SpectraMax DuoLuc assay in 96- and 384-well formats with high sensitivity and throughput. of Dispensers:Molecular Devices deliver high throughput while showing good correlation with manual. 300 µL of Detergent Compatible Bradford reagent was added to each well. Important things to consider when purchasing a multimode microplate reader. The onboard luminescence detection mode may be used, or, for higher sensitivity, the reader can be equipped with a NanoBRET detection cartridge that offers a 4-fold improvement in limit of quantitation. The StakMax stacker also allows users to feed plates into the loading area manually. 314 low lamp level warning. x. SoftMax Pro GxP, together with a Molecular Devices plate reader and Molecular Devices GxP Admin software, provides a fully integrated hardware and software solution forThis UV-visible absorbance microplate reader provides ultrafast, full spectral range detection for cuvettes, 96-well, and 384-well microplates, with the only temperature-independent method for pathlength correction. By measuring optical pathlength, it eliminates the last major distinction between microplate readers and spectrophotometers. Read the plate on the SpectraMax M5, M5e, or FlexStation 3 reader using the optimized settings shown above. % transmittance of plates used in a plate reader. SpectraMax 250 User. SoftMax Pro GxP Software version 5. Primary considerations when choosing a microplate reader are throughput and flexibility. 96-well plate: 0 to 4 Abs, ±2% at 450 nm, 384-well plate: 0 to 3 Abs ±2% at 450 nm: Measurement Type: Endpoint, Kinetic, Spectra, Multipoint, Kinetic Spectra: No. Users can choose to process all plates within the magazine or select a specific number. The SpectraMax® M5 Multi-Mode Microplate Reader delivers single mode reader performance and can be equipped to read volumes as low as 2uL in one multimode reader package. Deep well blocks available in 24, 96 and 384-well formats An affordable, tunable microplate reader for 96-well format Affordable The robot-compatible VersaMax reader is cost-effective—users never have to buy filters. Upgradeable modules are also available including western blot, cell imaging, and fast kinetics with injectors. It provides ready-to-run protocols, analysis algorithms, and 21 different curve fit options. 384-WELL SBS STERILE MICROPLATE. SpectraMax® Absorbance Microplate Readers can run both cuvette-based and microplate reader applications on the same instrument. None for any other plate (6, 12, 24, 48 well plates) Flex3: Both Top read and Bottom read. #C1500) Control standard endotoxin (ACC cat. The procedures in this protocol support the 96-well reaction plate. 5°C at 37°C. Features. SpectraMax ID5 Plate Reader (Molecular Devices). Products : General, FilterMax F5 reader. Features. A: adipocytes; B: fibroblasts; C: no cells (uptake reagent only). 000 to 2. Temperature control: 4°C above ambient to 45°C. #25-340) Pyrogen-free Dilution Tubes (Lonza cat. The LysoTracker fluorescence at ex/em 574/594 was measured on a Spectramax i3x plate reader (Molecular Devices). Optimized for fluorescence microplate readers. 1. Before dispensing reagents to the microplate, the VersaMax Reader was powered on and SoftMax Pro GxP. Populate the following info under Description. Selects either 96-well or 384-well plate for a stand-alone read. S. Computer Integration Each Molecular Devices microplate reader is shipped with a license key for the SoftMax® Pro Data Acquisition and Analysis Software that you install on the computer that you use to operate the instrument. The AquaMax® Microplate Washer is a fully self-contained system, configurable for both 96- and 384-well microplates. Corning. The Molecular Devices Gemini EM is built on the Gemini platform delivering high quality top and bottom reading for both solution and cell based assays. Select models. Each well of a 96- well microtiter plate is coated with a fluorescently labeled IgG-binding peptide, Protein G. However, for greater flexibility, some instruments can accommodate 6- to 1536-well plate formats. It features a modular design that allows users to add or change detection cartridges according to their needs. Absorbance values were read on the SpectraMax iD5 reader using a preconfigured BCA assay protocol (the SpectraMax iD3 reader is also suitable for these assays, as it has the same absorbance performance as the SpectraMax iD5 reader). Dimensions: 18. Title: SpectraMax i Series Multi-Mode Microplate Readers | Molecular Devices Author: Molecular Devices Subject: SpectraMax i Series offer the ideal solution for any application whether you are looking for an intuitive, easy-to-use reader, multi-user lab, or an upgradeable system that can adapt to support your lab s new projects. For a higher degree of automation, the Automation Vendor Partners Program has streamlined the integration of our microplate reader systems with all leading partner robots. Two different agonists, acetylcholine and carbachol, were tested. 25, 3. 9. 1 fmol luciferase per well. (96- and 384-well with pipetting) 6-384 (96- and 384-well). Packed 25 plates per…. Resipher 96x Starter Kit for use with 96-well plates Includes: (1) Resipher Data Hub, (1) Resipher 96x Reader, (3) sterile sensing lids, all cables Turns a 96-well plate into a precise dynamic oxygen consumption reader, measures 96 wells Utilizes Lucid Lab Cloud software for connection to any…. We have the following plate-reader instrument available for on-site screens and other projects Molecular Devices Spectramax Paradigm for luminescence, fluorescence, and. BioTek Instruments Synergy Neo2 Multi-Mode Reader Fluorescence Measuring Equipment. VersaMax Microplate Spectrophotometer Operator's Manual Molecular Devices Corporation 1311 Orleans Drive Sunnyvale, California 94089 Part # 0112-0126 Rev A. FAST AND ACCURATE METERTECH Microplate Reader $3,650. Shaking: Linear, orbital, and double orbital. 3% in white 96- and 384-well microplates < 3 x 10-5 (white plates) < 3 x. Measurements for every well are automatically tracked over time. #ABS Plus) Cell plating and treatment prior to assay. An additional reference channel for optimizing the light intensity; Auto switch-off of the light source when not in use to extend lifetime; An automated self-check of major components at instrument start-upThe EMax® Plus Microplate Reader delivers a robust solution in an entry level platform. 4 (Molecular Devices cat. At 5 seconds to read a 96-well endpoint assay or a 5 second minimum interval between kinetic reads the VMax Microplate Reader has both speed and performance. ©2017 Molecular Devices, LLC 3/17 0120-1441I Printed in USA Figure 3. #R8195. Published Dec 16, 2020 | Updated Dec 16,. Published Dec 16, 2020 | Updated Dec 06, 2021. Washer maintenance kit: 2 X T 2. The color touch screen provides a visual user interface, making programming fast and intuitive.