314 low lamp level warning. Molecular Devices SpectraMAX 340 Microplate Reader. Narrow bandwidths deliver increased measurement accuracy and linearity over a wide range of assays. 3 0. All variables were tested in three independent cultures for each experiment, which was repeated twice (n = 6). The ABS reader ranges from. Molecular Devices SpectraMax Gemini XS Microplate Reader. 00 USD. The microtiter plate was then incubated in the dark for 2 h at 37°C. The SpectraMax® QuickDrop™ Micro-Volume Spectrophotometer . Wavelength range: 340-850 nm. The drawer will open and it may close, if is doesn't, close it using the DRAWER button found on the Spectramax control panel (pictured in purple on. Alkaline phosphatase (ALP) activity. density was measured at 570 nm (SpectraMax 340, Molecular De-vices, Sunnyvale, CA). Time-resolved fluorescence was measured on the SpectraMax i3x and SpectraMax iD5 readers using preconfigured protocols in SoftMax® Pro Software (see Tables 2 and 3 for instrument-specific settings). The chamber contains. 313 reference gain check fail. reader (SpectraMax 340; Molecular devices, LLc). 0112-0126B 8 Chapter1:Introduction TheSpectraMax®340PC384,SpectraMax®190,andVersaMax™microplate. Values are expressed as the mean s ± SD of 3 separate experiments, each performed in triplicate. Read one sample or up to 384. Revvity. Molecular Devices SpectraMax M5e Microplate Reader. Briefly, hPDLSCs were cultured in osteogenic/odontogenic induction medium for 14. Description 1. Male 8 pin mini Din to Female 9D. 003 OD Stray light ≤ 0. A 0. Please see photos. Detection limits. SpectraMax Multi-Mode Microplate Readers. BV-2 cells were treated with various concentrations of BT and/or LPS for 24 hFollowing incubation, absorbance was immediately measured at 405 nm with a SpectraMax 340 spectrophotometer (Molecular Devices, LLC. Details. This is equivalent to having 511 built-in filters,Results: A total of 64% of the subjects reported improved quality of sleep during treatment with naloxone at a dose of 40 ng per 24 hours as compared with 9% with sham treatment (P=0. For 40 years, we have partnered with scientists to help them achieve landmark discoveries. Top and bottom reads are available for fluorescence, time-resolved fluorescence and luminescence detection. Top and bottom reads are available for fluorescence, ti me-resolved fluorescence and luminescence detection. When reading at wavelengths below 340 nm, special UV-transparent, disposable or quartz microplates and cuvettes that. Values were determined according to a standard curve (log/log curve-fit). . 2. Below is the list of Molecular Devices microplate readers and liquid handling instruments that are of out-of-production and are now considered obsolete. The SpectraMax M2 is a dual-monochromator, multi-detection microplate reader with a dual-mode cuvette port and 96 or 384 microplate reading capability. , Sunnyvale, CA, USA) at 595 nm. Molecular Devices Spectramax 340PC instructions. ISM Equipment Classification (Group 1, Class A) This equipment is designated as. Our multi-mode plate readers provide great flexibility and include absorbance, fluorescence and luminescence with configurable options for fluorescence polarization (FP), time-resolved fluorescence (TRF), FRET, and AlphaScreen. SpectraMax 340 from Molecular Devices). Lightgreenglass,2 mmthick(340 nm) 9:Yellowglass,3 mmthick(405 nm) 10:Darkredglass,3 mmthick(650 nm)automated SpectraMAX 340 microtiter plate reader. Incubation with TMB substrate was done for 18 min. When reading at wavelengths below 340 nm, special UV-transparent, disposable or quartz microplates and cuvettes that allow transmission of the far UV spectra must be used. The SpectraMax® Plus 384 Microplate Reader from Molecular Devices can run both standard spectrophotometer and microplate reader applications on the same instrument. The manufacturer of the SpectraMax 340PC Microplate Spectrophotometer is Molecular Devices. LAL Reagent Grade ™ Multi-well Plates (Lonza cat. 2. This assay measures the two major sugars in wine, glucose and fructose. Not all manufacturers' microplates are the same with regard to design, materials, orIn a typical 100-μL assay with mixtures containing 50 mM MOPS, pH 6. Absorbance was recorded at 540 nm using SpectraMAX 340 reader. Afterwards, absorbance at 595 nm was measured with spectrophotometer (SpectraMax 340, Molecular Devices, USA). The activity of generated thrombin was measured by the amidolysis of its substrate S-2238 and theThe purple formazan crystals formed were dissolved by adding 100 μL of DMSO to each well and absorbance was read at 570 nm in a spectrophotometer [SpectraMax 340]. The intensity of red color formed in the assay was measured at a wavelength of 490 nm with an ELISA reader (SpectraMax 340, Molecular Device Co. Labsystems Luminoskan RT Microplate Reader. The absorbances were being measured at 515 nm. The inhibition of ALDH activity by compounds and the IC50 curves were determined by measuring the formation of NAD(P)H spectrophotometrically at 340 nm (molar extinction coefficient of 6200 M −1 cm −1) on a Beckman DU-640 as well as a Spectramax 340 PC spectrophotometer (GMI, Ramsey, NJ, USA) using a purified. Liquid handling for random screening was carried out with a Biomek FX from Beckman Coulter (Fullerton, CA, USA) and HYTRA. When reading optical density at wavelengths below 340 nm, special UVtransparent, disposable or quartz microplates and cuvettes that allow transmission of the far UV spectra must be used. The assay was carried out in a 96-well plate in a final volume of 100 µl containing 50 mmol/l Tris-HCl, pH 7. The Molecular Devices SpectraMax 340PC384 Microplate Reader can run both standard spectrophotometer and microplate reader applications on the same instrument. Intra- and inter-assay coefficients of variation were lower than 8. Other technologies such as imaging. Molecular Devices SpectraMax 340 PC 384 Microplate Reader. 04. Quick View. Money Saved: $340. SpectraMax® 340PC384 microplate reader provides everything needed to measure absorbance in the visible range, including temperature. The mixture changed to a red color by a reaction of LDH that was measured by a plate reader (SpectraMax 340 PC; Molecular Devices, San Jose, CA, USA) at a visible wavelength of. Awareness Technology, Inc. 0 3. The membranes were. SpectraMax 340PC 384 and SpectraMax Plus 384 read both 96-and 384-well microplates. 8 (w) x 15 (d) Performance Specifications Wavelength Range: 340 – 850 nm Wavelength Selection: Monochromator, tunable 1. Apoptosis was also measured by terminal deoxynucleotidyl transferase-mediated dUTP-biotin nick end-labeling (TUNEL) assay using the TACS Blue label kit (4811-30-K, R&D Systems). With SOFTmax PRO, the contents of the wells in a microplate can be mixed the industry-leading microplate reader software for the SpectraMax Plus 384 system, allows complete customization of data collection and analysis. Statistical analysis. 0 to 4. 314 low lamp level warning. 0112-0115G October2018 SpectraMax®M3 SpectraMax®M4 SpectraMax®M5 SpectraMax®M5e Multi-ModeMicroplateReaders UserGuideMolecular Devices SpectraMax Gemini XS Microplate Fluorometer $2,800. Both readers feature an 8-channel read head for fast reads and. TGFα release was calculated as described in the Results. 1 0 100 80 60 40 20 0 4. Fully tested with Warranty. 340 to 740 nm (linear variable dichroic) 220 to 1000 nm for abs (spectrometer) Plate Formats: 6 to 384-well plates, LVis Plate with sixteen 2 µL microspots. This tunable visible absorbance reader is for 96- and 384-well microplates and acquires data in endpoint, kinetic, and spectral scan modes using wavelengths from 340-850 nm, tunable in 1. SpectraMax M2, while top and bottom reads are possible on the SpectraMax M2 e. PerkinElmer. 97), and each plate contained at least six positive and six negative controls. Wells were washed three times. Features demonstrated by the model include temperature control, a robot-compatible interface and data analysis software. SPECTRAmax 340PC Microplate Spectrophotometer Operator’s Manual 1-3 Introduction General Overview The SPECTRAmax ® 340PC incorporates a holographic grating. Cell permeabilization. -1 activity was determined MMP using an MMP-1 immunoassay kit (dY901; R&d Systems, Inc. Capabilities include fluorescence and luminescence readings, environmental control, cell viability assays. SpectraMax Multi-Mode Microplate Readers. Detection modalities include absorbance (UV-Vis Abs) and fluorescence intensity (FI). 2 g/L solution; dilute 1:10 in HBSS. Wavelength Selection. Absorbance was read at 450 nm using a Molecular Device Spectramax 340. 2 GxP Software is the latest, most secure software to achieve full FDA 21 CFR Part 11 and EudraLex Annex 11 compliance with. When you do reads at wavelengths below 340 nm, you must use special UV-transparent, disposable or quartz plates to allow transmission of the deep UV spectra. 4. By using PathCheck, SpectraMax readers can automatically normalize sample absorbance values to a 1-cm pathlength and calculate the concentrations. This tunable visible absorbance reader is for 96- and 384-well microplates and acquires data in endpoint, kinetic, and spectral scan modes using wavelengths from 340-850 nm, tunable in 1. These robust readers have been placed in labs from Antarctica to the. The plate was covered and incubated for one hour at room temperature. Number of flashes. 312 gain calibration failed. The fluorescence intensity was measured using the SpectraMax 340 microplate reader (Molecular Devices) at the following conditions: excitation at 475 nm and emission at 529 nm (green fluorescence) or 590 nm (red fluorescence). Microplate Spectrophotometer Operator’s Manual. Finally, the absorbance at 450 nM was taken using a SpectraMax ® 340 ELISA plate reader (Molecular Devices, USA). , Fremont, CA, USA). The increase in fluorescence was determined for 20 min by excitation at 520 nm and emission at 545 nm in a. The absorbance at 540 nm was determined in a spectrophotometer (SPECTRAmax 340, Molecular Devices). The SpectraMax i3x reader provides the flexibility to add novel detection capabilities without the need for service engineers or costly downtime—it grows with you as your research areas expand. 3 mm. Molecular Device M2e. Glutathione reductase (GR) activity was measured, according to Smith et al. The Molecular Devices SpectraMax 340PC384 Microplate Reader can run both standard spectrophotometer and microplate reader applications on the same instrument. The estimation of monodehydroascorbate reductase (MDHAR) (EC 1. The SpectraMax® ABS and ABS Plus absorbance microplate readers provide the flexibility, sensitivity, and convenience for a wide range of assays such as ELISAs, microbial growth, and protein quantitation. SpectraMax 340PC384 reads 96-well plates and 384-well plates and strip-well plates. See More Microplate Reader / Microtiter Plate Reader. The contents of the wells in a microplate can be mixed automatically by shaking before each read cycle, which makes it possible to perform kinetic analysis of solid- Absorbance at 405 nm (Abs 405) was obtained for both plates before (background) and after 1-h incubation at 37°C using a microplate reader (SpectraMax 340 PC384, Molecular Devices). SPECTRAmax™ 340 (Molecular Device, CA, USA) at 37 ° C for 30 min. Includes: Computer Loaded with Softmax Pro 5. The SpectraMax iD5 reader uses the HTRF detection system, which includes an Enhanced TRF Module and filters for excitation and emission. Upgradeable modules are also available including western blot, cell imaging, and fast. Briefly, ear or dorsal skin was dissected and fixed with 10% phosphate buffered formalin for 24–72 h. 1% Chaps, 10 m M dithiothreitol, and 10% sucrose. Stat Fax 4700 Microstrip Reader. Following cell culture under normoxic or hypoxic con-ditions, culture medium samples were obtained on completing the incubation period to measure atrial natriuretic peptide. Proteolytic activity was determined using the synthetic chromogenic substrate Nα-benzoyl-DL-arginine p-nitroanilide (DL-BApNA), in 96-well plates, according to Ponce-Soto et al. 0112-0126B 8 Chapter1:Introduction TheSpectraMax®340PC384,SpectraMax®190,andVersaMax™microplate. The screening was initiated by adding 10 μL p-nitrophenyl phosphate (pNPP), and the catalysis of pNPP (1 mM) was continuously monitored on SpectraMax 340 microplate reader (Molecular Devices, Sunnyvale, CA, USA) at 405 nm for 20 min at 37 °C. Temperature Range: Ambient + 4 °C up to 45 °C. When reading optical density at wavelengths below 340 nm, special UV-transparent, disposable or quartz microplates and cuvettes that allow transmission of the far UV spectra must be used. \r plates can be used in the SPECTRAmax PLUS 384. Samples were plated in duplicate (r=0. 8–250 nM) was measured as follows: the prothrombinase reaction proceeded for 3 min and was stopped by the addition of 10 mM EDTA. 30. IC 50 values were determined by mathematical curve-fitting (SoftMaxPro Molecular Devices) and were defined as the drug concentration leading to 50% inhibition of net cell mass. Details. 2. Figure 1. Near bottom. Read up to 6 wavelengths in endpoint or kinetic mode for 96- and 384-well plates, and perform spectral scans to determine. Tunable for filterless flexibility. Our absorbance plate readers feature our PathCheck Sensor technology and. Our microplate readers and software are the industry’s most cited and have empowered life science researchers to advance protein and cell biology. SpectraMax 384 Plus. Analysis of ALA by Ehrlich’s Reagent Assay. Molecular Devices SpectraMax Gemini XS Microplate Reader. Nitric Oxide Assay. Stat Fax 4700 Microstrip Reader. (7. SpectraMax i3/i3x and Paradigm readers can be equipped with the HTRF detection cartridge. Isolation of RNA and RT-PCR. Read one sample or up to 384 in a single plate read using any standard cuvette, 12 x 75 mm test tube, or 96- or 384-well microplate. After adding the stop solution, the absorbance at 450 nm with a wavelength correction at 540 nm was measured with a SpectraMax 340 plate reader (Molecular Devices, San. Then, the absorbance value at 450 nm was determined using a microplate reader (SpectraMAX 340; Molecular Devices, Sunnyvale, CA, United States). SpectraMAX 340 microtiter plate reader. In vivo anti-malarial assay in mice. 316 grating motor driver faulty. used. 00. Manual: SoftMaxPro User Guide Manufacturer: Molecular Devices Location: SOM-E536. Microplate Readers. Service Contracts, Preventative Maintenance and Performance. SpectraMax® Gemini™ EM Microplate Reader, Molecular Devices. Urinary proteins were measured using a Micro BCA assay kit following the manufacturer’s instructions (Thermo Fisher Scientific, Rockford, IL, USA). SpectraMax M2, while top and bottom reads are possible on the SpectraMax M2 e. Details. , Sunnyvale, CA) after incubation of solution mixture at 37 °C for 2 h 30 min in the dark. Molecular Devices SpectraMax 384 Plus Microplate Reader Specifications. 5. 6, 2 mmol/l DTT, 10 mmol/l MnCl 2 and 10 mmol/l pNPP; the PP2Cα enzyme concentration was 400nmol/l. , Sunnyvale, CA). When reading optical density at wavelengths below 340 nm, special UV-transparent, disposable or quartz microplates allowing transmission of the deep UV spectra must be used. Molecular Devices SpectraMax M5e Microplate Reader. It includes both a lamp and a new lamp holder. Analysis of ALA by Ehrlich’s Reagent Assay. 0% and ± 0. Subsequently, the mixture was heated at 100 °C for 10. . Cytotoxicity was determined by measuring the release of LDH. Media in plates were withdrawn by pipetting and 200 μl of DMSO was added to each well to solubilize the formazan crystals. Quick View. Page: 1/6. 5 g/L-EDTA 0. Photometric Range: 0. Whether you are looking for an intuitive, easy-to-use reader for your busy, multi-user lab, or an upgradeable system that can adapt to support your lab’s new projects andSPECTRAmax ® PLUS 384 Microplate Spectrophotometer. 0. Spectral Range. Cell Assay Cells (5 × 10 5 per well) are plated in triplicate in 96-well plates in 0. Acceptor filter: 665/10 nm. The SpectraMax® absorbance spectrophotometers and plate readers provide the versatility and convenience for a wide range of assays such as ELISAs, nucleic acid and protein quantitation, and microbial growth. It measures the optical density (OD) of samples in both 96- and 384-well microplates at a selected wavelength for a single point in time (endpoint), over a specified period of time. Microplate Readers. Includes: SpectraMax 340PC384. LAL Reagent Grade ™ Multi-well Plates (Lonza cat. SPECTRAmax® 190. High fg levels of Streptavidin High fg levels of Streptavidin Exceeds Cisbio certification requirements TRF-based with 340/80 nm EX and 616/10 nm EM AlphaScreen AlphaScreen and AlphaLisa detection using 1 W 680 nm. Samples were considered “positive” if the absorbance value was greater than two times the average absorbance value of the negative control wells . FACS analysis. Create custom fluorescence assays without having to purchase expensive filter sets. gp70 blocking. 1 mL of acetylacetone. The activity of glutathione peroxidase (GPX) was quantified spectrophotometrically at 340 nm, following the method of Takeda et al. plates can be used in the SPECTRAmax PLUS 384. Each experiment was performed in duplicate and repeated at. The cytotoxicity of the CDDP loaded CHAMs were done by treating the FaDu cells with DMEM supplemented with 0. #ABS PLUS) Methods. 4; 100 m M NaCl, 0. The concentration of TNFα in the experimental. Column chromatography was performed on silica gel (Kieselgel 60, 70–230 mesh, Merck). 190-1000 nm, tunable in 1 nm increments. Homeostatic model assessment for insulin. SpectraMax and VersaMax Plate Readers Operator’s Manual — 0112-0126 Rev A 3 1. Example: [Drawer]. The SpectraMax ABS Plus, can accommodate standard 96-well plates and 384-well plates. ” SpectraMax® Quant™ dsDNA Assay Kits The SpectraMax® Quant™ dsDNA Assay Kits are designed for fluorescence-based dsDNA quantitation across a broad range of concentrations. Not all manufacturers' microplates are the same with regard to design, materials, orSpectraMaxABS Plus,SpectraMaxM2,SpectraMaxM2e,SpectraMax M3,SpectraMaxM4,SpectraMaxM5,SpectraMaxM5e,SpectraMaxPlus 384 0200-7200 Multi-Mode ValidationPlate. 340 nm. With SOFTmax PRO, the contents of the wells in a microplate can be mixed automatically by shaking before each read cycle, making it possible to. Table 1. Call 1-800-745-2710. 2. The values were corrected for nonspecific background by subtracting the OD 650. The VersaMax and SpectraMax 190 read 96-well microplates. We selected the response at t=10 h for statistical analysis, because at that time point the response variation was largest. Continuous kinetic monitoring of enzyme activity was performed on SPECTRAmax 340 or Flexstation 2-384 microplate reader (Molecular Devices, Sunnyvale, CA, USA) and controlled by the Softmax software. The absorbance was measured at 340 nm for 2 min at a 15 s time interval. The supernatants were mixed with an LDH assay reagent, which was purchased from Takara Bio Inc. The SpectraMax ABS/ABS Plus Microplate Readers provide fast absorbance detection without the use of filters with monochromator-based wavelength selection for visible and. Standard features include a cuvette port, spectral scanning in 1 nm increments, and up to six wavelengths per read. 4. , Sunnyvale, CA) after incubation of solution mixture at 37 °C for 2 h 30 min in the dark. 001/min at 425 nm. 2 - 3. Incubate 30 min at room temperature, and then read microplates at 405 nm using SpectraMax 340 PC plate reader (Molecular Devices) (see Note 8). The cell death was calculated as follows:Absorbance was monitored at 450 nm (SpectraMax 340). 2 were considered negative for IgM antibody. Exhibit with us {{>currencyLabel}} Back. These lab workhorses are used in drug discovery, research, bioassay validation, and. Bioz Stars score: 86/100, based on 1 PubMed citations. Optical density was measured at 570 nm using a microplate reader (SpectraMAX 340, Molecular Devices Co. A microplate reader that can detect two or more applications is considered a multi-mode plate reader. Subsequently, the oxidative burst was initiated by the addition of PMA (final concentration 100 nM) and the cells incubated for 120 min at 37°C. The increase in fluorescence was determined for 20 min by excitation at 520 nm and emission at 545 nm in a. Irene Tieleman, Ido PenandSimon VerhulstSubsequently, the oxidative burst was initiated by the addition of PMA (final concentration 100 nM) and the cells incubated for 120 min at 37°C. WST regents of 10% were added to each well, and absorbance was determined at 450 nm using an ELISA reader (SpectraMax® 340, Molecular Device Co. Not all manufacturers' microplates are the same with regard to design, materials, or76358-624. A. 0, MSD) or Spectramax 340 (Molecular Devices). After 24 h, 48 h, 72 h or 96 h, cell viability was measured by CellTiter 96® AQueous One Solution cell proliferation assay (Promega, Madison, WI, USA). Gerritsma,B. Then the samples were incubated for 1 h with human IL6 detecting antibody and then with HRP conjugate for 40 min. Changes in cell viability were reflected by changes in optical density detected at 450 nm, which was measured using a spectrophotometer microplate reader (SpectraMax 340; Molecular Devices, Sunnyvale, CA, USA). The light features a slim profile to prevent. SPECTRAmax 340PC Microplate Spectrophotometer Operator’s Manual 1-3 Introduction General Overview The SPECTRAmax ® 340PC incorporates a holographic grating monochromator which allows you to specify a precise wavelength, from 340 nm to 850 nm, for the absorbance maximum of your sample. well, and strip-well microplates. 1 mM MnCl2 and 25 mM CHES buffer, pH 9. With a wide range of on-board features and capabilities, thermoax is ideally suited for use in modern laboratories involved in many applications. For cell permeabilization, 1 ml of Z buffer (60 mM Na 2 HPO 4 z 7H 2 O, 40 mM NaH 2 PO 4 z H 2 O, 10 mM KCl. the SpectraMax iD3 is a fantastic plate reader for a wide range of assays. The SpectraMax ABS readers use a grating monochromator to select the exact wavelength needed for every assay. MCE has not independently confirmed the accuracy of these methods. 260 280 300 320 340 360 Instrument: Molecular Devices SpectraMax 250 Instrument: LAMBDA 19/UV/VIS/NIR O. , Sunnyvale, CA, USA) at a wavelength of 450 nm. 9000-0756. VersaMax reads 96-well and strip-well plates. 6, 2 mmol/l DTT, 10 mmol/l MnCl 2 and 10 mmol/l pNPP; the PP2Cα enzyme concentration was 400nmol/l. DNA/RNA concentration is commonly estimated by dividing the A260 value by the 1-cm absorptivity value (or multiplying by its reciprocal). Finally, after another 45 min of decolorization, 100 µL of the solution was transferred to a new well and the amount of crystal-violet stain in the decolorizing solution was measured with a microtiter plate reader (SpectraMAX 340 Adjustable Microplate Reader; Molecular Devices Ltd. All strains were tested three times and the results averaged. 5 - 2. As a common indicator of equine T cell activation, cytokine induction. The first time you replace an OEM lamp you must use the N-34-MLA. The SPECTRAmax 340PC can measure. Spectrophotometer (SpectraMax 340). When reading at wavelengths below 340 nm, special UV-transparent, disposable or quartz microplates allowing transmission of the deepThe SpectraMax i3x reader measures spectral-based absorbance, fluorescence, and luminescence with the added functionality of modular upgrades for western blot, imaging, and fast kinetics with injectors. 5. Stat Fax 3200 Microplate Reader. Molecular Devices Spectramax 340PC instructions. reader (SPECTRAmax™340; Molecular Devices, Palo Alto, CA, USA). The Analyst®, FlexStation® and SpectraMax® M5/M5 e from Molecular Devices have received the LanthaScreen® Certified designation from Life Technologies which ensures that these readers are validated to strict standards in instrument setup and assay performance. The. Absorbance measurements were recorded using a SpectraMax 340 (Molecular Devices) at 570nm on the indicated days (Cell Proliferation Kit I; Roche). 0200-7027. No power cord or accessories included. TheSpectraMax®340PC384,SpectraMax®190,andVersaMax™microplate spectrophotometersproviderapidandsensitivemeasurementsofavarietyofanalytes. Developed plates were read on MSD reader Sector Imager S 600, Model 1200, with Discovery Workbench Software (version 4. With optimized reagents and the industry leading data acquisition and analysis tool, SoftMax® Pro 7 Software, the SpectraMax to perfectly match your needs. Description Control Panel Cuvette Chamber Back Panel Microplate Drawer The Control Panel TEMP on/off TEMP Figure 1. A 0. Amino Acid Analysis. Each experiment was performed in duplicate. Up for your consideration is a Molecular Devices SpectraMax 340 Microplate Spectrophotometer Model# 340 Condition is for parts or repair. (Shiga, Japan) and kept for 30 min in the dark. Quick View. The centrifuged solution was transferred to a different microtitre plate and the optical density (OD) of the change in color was measured using a plate reader (SpectraMAX 340 Tunable Microplate Reader; Molecular Devices Ltd. One plate carrier adapter is provided with the instrument. When reading at wavelengths below 340 nm, special UV-transparent, disposable or quartz microplates and cuvettes that allow transmission of the far UV spectra must be used. using a Molecular Dynamics Model SpectraMAX 340 PC microplate reader. All assays were performed in triplicate, and the absorbance at 405 nm was measured using a SpectraMax 340 multiwell plate reader (Molecular Devices). ). , Sunnyvale, CA). Molecular Devices SPECTRAmax. With a wide range of on-board features and capabilities, thermoax is ideally suited for use in modern laboratories involved in many applications. The SpectraMax® M Series Multi-Mode Microplate Readers are modular and upgradeable with a wide range of high performance capabilities ideal for life science research and drug discovery screening SoftMax® Pro 7. The extinction coefficient at 280 nm used for determining the concentration of Rsp3690 was calculated to be 28,795 M –1 cm –1. Located at the right front of the SpectraMax Plus 384, the cuvette chamber has a lid that lifts up, allowing you to insert or remove a cuvette. Introduction The SpectraMax® 340PC384 Reader from Molecular Devices provides everything needed to measure absorbance in the visible range, including temperature control, a robotics-compatible interface and SoftMax® Pro Data Acquisition and Analysis Software. For use with Spectramax 340 devices which have an 8-pin mini DIN serial socket. Absorbance was measured at 490/650 nm by using a SpectraMax 340 microplate spectrophotometer® (Molecular Devices, Sunnyvale, CA, U. Briefly, cells were seeded into a. albicans pre. 024). 306 plate air cal fail,low light. Call 1-800-745-2710. Call 1-800-745-2710. The optical system of the SpectraMax 340PC Microplate Reader is built around a monochromator, which allows for selection of up to six wavelengths at a time for absorbance detection in the visible wavelength range (340 nm - 850 nm). or microplate reader (e. GE Healthcare spectramax 340 pc plate reader Spectramax 340 Pc Plate Reader, supplied by GE Healthcare, used in various techniques. 2 software was used to measure and calculate of the bioactivity activity studies. Oxidative stress induction by H 2 O. 5), L-ascorbic acid (25. Binding affinity for denosumab. Absorbance values were read at 570 nm on an automated SpectraMAX 340 (Molecular Devices, Sunnyvale, CA, USA). Background: Ketamine can act as a multifunctional neuroprotective agent by inhibiting oxidative stress, cellular dysfunction, and apoptosis. Fibrin clots were formed as described above with some modifications 46. Media in plates were withdrawn by pipetting and 200 μl of DMSO was added to each well to solubilize the formazan crystals. Operators can conveniently select up to 6 wavelengths at a time for. The reaction was then stopped by adding 50 µL of 1 M sulfuric acid to each well. P. Read up to 6 wavelengths in endpoint or kinetic mode for 96- and 384-well plates, and perform spectral scans to determine. Top and bottom reads are available for fluorescence, ti me-resolved fluorescence and luminescence detection. Awareness Technology Inc. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the. Alkaline phosphatase (ALP) staining assay ALP activity was detected using a SensoLyteVR pNPP Alkaline Phosphatase Assay Kit (AnaSpec, Inc. 5 hours, and is measured in a 96-well plate spectrophotometer at 405 nm (SpectraMax 340; Molecular Devices LLC, Sunnyvale, CA, USA). Manual: SoftMaxPro User Guide Manufacturer: Molecular Devices Location: SOM-E536 The SpectraMax® i3x Multi-Mode Microplate Reader measures absorbance, fluorescence, and luminescence with available upgrades including western blot, cell imaging, and fast kinetics with injectors plus additional detection modes. The SpectraMax® 190 Absorbance Microplate Reader is the ideal equipment for many life science applications including DNA analysis.