340/70 nm. A microplate reader asThe absorbance was measured at 425 nm and read every 10 min using a Spectramax 340 plate reader (Molecular Devices ®, USA). Cells were scraped from the plate and single cell suspensions were made by passing cells through 28G1/2 insulin syringes. Hydrolysis of compounds 9-15 was monitoredAbsorption was measured at 405 nm against 490 nm using a microplate reader (SPECTRAmax™340; Molecular Devices, Palo Alto, CA, USA). chen@case. Intra- and inter-assay coefficients of variation were lower than 8. 6) and 0. 340/80 nm excitation with 616/10 nm emission range Optimized for use with ScanLater Western Blot Detection cartridge for the SpectraMax® i3, i3x, and Paradigm® Multi-Mode Readers Optimized for use with the. Capabilities include fluorescence and luminescence readings, environmental control, cell viability assays. The SpectraMax® 340 PC 384 Absorbance Microplate Reader from Molecular Devices provides the necessary tools for absorbance measurement in the visible range. 2. The SpectraTest validation package provides a NIST-traceable solution for validating optical performance of the SpectraMax Plus 384 reader and does it automatically. 5 mL of 1 M acetate buffer (pH 4. TheSpectraMax®190MicroplateSpectrophotometer,SpectraMax®340PC384Microplate Spectrophotometer,andVersaMax™MicroplateSpectrophotometerproviderapidandsensitive. After 24 h, 48 h, 72 h or 96 h, cell viability was measured by CellTiter 96® AQueous One Solution cell proliferation assay (Promega, Madison, WI, USA). Anti-proteolytic activity. TheSpectraMax®340PC384,SpectraMax®190,andVersaMax™microplate spectrophotometersproviderapidandsensitivemeasurementsofavarietyofanalytesacross awiderangeofconcentrations. Top and bottom reads are available for fluorescence, time-resolved fluorescence and luminescence detection. The concentration of NO in the cells was determined by measuring the amount of nitrite, a relatively stable oxidation product of. SpectraMax 250 With Computer SoftwareSpectraMax 340PC384 Microplate Reader A versatile 96- and 384-well format absorbance reader let the monochromator tune to that exact wavelength. 190–850 nm <± 1. Each SpectraMax® 340 PC 384 Microplate Reader has an. 316 grating motor driver faulty. Softmax PRO v5. All absorbance measurements were taken on a SpectraMax 340 PC reader equipped with SoFTMax Pro software. below 340 nm, special UV-transparent, disposable or quartz microplates and cuvettes that allow transmission of the far UV spectra must be used. 5. 5. 8 (w) x 15 (d) Performance Specifications Wavelength Range: 340 – 850 nm Wavelength Selection: Monochromator, tunable 1. The drawer will open and it may close, if is doesn't, close it using the DRAWER button found on the Spectramax control panel (pictured in purple on. The estimation of monodehydroascorbate reductase (MDHAR) (EC 1. Quick View. The SpectraMax® 190 Absorbance Microplate Reader is the ideal equipment for many life science applications including DNA analysis. Create custom fluorescence assays without having to purchase expensive filter sets. Created Date: 20051213090753Zmeasured using a SpectraMax 340 multiwell plate. LAL Reagent Grade ™ Multi-well Plates (Lonza cat. NHDFs were seeded in microtiter plates (96 wells) at a density of 1x105 cells per well. TDP-43 stress granular cells were treated with 70% ice-cold. SpectraMax® M2/M2e user guide a multi-detection microplate reader with two-mode cuvette port SpectraMax M2e user guide cover 1 1 4/21/06 9:53:28 AM. Absorbance was then measured at 540 nm (isosbestic point of cytochrome c) and 550 nm (SpectraMax 340; Molecular Devices, Sunnyvale, CA, USA). 7 macrophages were obtained from ATCC (Manassas, VA). TDP-43 stress granular cells were treated with 70% ice-cold. Cell viability was determined using the blue formazan assay in which colorless 3-(4,5-dimethyl-thiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) is metabolized to a blue product by mitochondrial dehydrogenases. All assays were performed using a sample size of 12, and the absorbance at 425 nm was measured by using a SpectraMax 340 multiwell plate reader (Molecular Devices, Sunnyvale, CA). When reading at wavelengths below 340 nm, special UV-transparent, disposable or quartz microplates and cuvettes that allow transmission of the. 8 pin mini DIN (Spectramax 340) to 9D (PC) serial data transfer cable. The GH Pro 340 Supplemental Greenhouse LED grow lighting is designed with greenhouse operations in mind. #25-340) Pyrogen-free Dilution Tubes (Lonza cat. SpectraMax and VersaMax Plate Readers Operator’s Manual — 0112-0126 Rev A 5 1. The drawer will open and it may close, if is doesn't, close it using the DRAWER button found on the Spectramax control panel (pictured in purple on. • TRF-based with 340/80 nm EX and 616/10 nm EM 0200-7027: EX range: 340/80 nm EM range: 616/10 nm: High fg levels of Streptavidin: High. Fios Router Setup Manual, Losi Xx4 We Manual, Statics Hibbeler Solutions Manual 12th Edition, Nokia Hs 23 Manual, Spectramax 340 Manual, Nolan N102 User Manual, Ship Security Officer Manual yunqian 4. Data were expressed as the mean percent viable cell vs. Our absorbance plate readers feature our PathCheck Sensor technology and. 0 nm increments Wavelength Bandwidth: 2 nm Wavelength Accuracy: < ± 1. Products : SpectraMax Plus 384 reader,. The absorbance was measured at 340 nm for 2 min at a 15 s time interval. Awareness Technology, Inc. Cytotoxicity was determined by measuring the release of LDH. #W50-100) SpectraMax® ABS Plus Microplate Reader (Molecular Devices cat. Narrow bandwidths deliver increased measurement accuracy and linearity over a wide range of assays. 0112-0115J 9 Chapter1:Introduction TheSpectraMax®M3Multi-ModeMicroplateReader,SpectraMax®M4Multi-ModeMicroplate Reader,SpectraMax®M5Multi-ModeMicroplateReader,andSpectraMax®M5eMulti-Mode Opening the Drawer Manually on page The SpectraMax ABS can accommodate standard 96-well plates. DNA/RNA concentration is commonly estimated by dividing the A260 value by the 1-cm absorptivity value (or multiplying by its reciprocal). TDP-43 stress granular cells were treated with 70% ice-cold. 6, 2 mmol/l DTT, 10 mmol/l MnCl 2 and 10 mmol/l pNPP; the PP2Cα enzyme concentration was 400nmol/l. In vivo anti-malarial assay in mice. 5 mm (bottom read) Beam diverges to focal spot near bottom of well. Page 2 1) All labor charges to repair the product for a period of one (1) year from the original date of. The extinction coefficient at 280 nm used for determining the concentration of Rsp3690 was calculated to be 28,795 M –1 cm –1. 315 can't find zero order. Data System. 0112-0126B 8 Chapter1:Introduction TheSpectraMax®340PC384,SpectraMax®190,andVersaMax™microplate. 001/min at 425 nm. 0 1. , Sunnyvale, CA). 303 unable to cal dark current. The reduction of HED in the presence of NADPH and GR was recorded at 340 nm. Cell proliferation was measured using the MTT method. Enzyme activities at lower concentrations were assayed by using a LS50B fluorometric. ). LDH release was measured with a commercial kit following the manufacturer’s instructions (LDH Cytotoxicity Detection Kit, TaKaRa). SpectraMax 340PC384 Microplate Reader A versatile 96- and 384-well format absorbance reader let the monochromator tune to that exact wavelength. 5. 2. Molecular Devices. 97), and each plate contained at least six positive and six negative controls. To. The activity of glutathione peroxidase (GPX) was quantified spectrophotometrically at 340 nm, following the method of Takeda et al. mums. Figure 1. The SpectraMax® ABS and ABS Plus absorbance microplate readers provide the flexibility, sensitivity, and convenience for a wide range of assays such as ELISAs, microbial growth, and protein quantitation. 0 Micromoles per Joule, ensuring the highest light output using the least amount of electricity. Absorbance was read at 405 nm on a microplate reader (SPECTRAmax 340 PC). On 1, 7, 14, and 21 days, the cells were washed with DPBS twice and incubated with CCK-8 solution for 2 h in the dark at 37 °C. Lactate Dehydrogenase (LDH) Release. Software (SoftMax Pro ® software or comparable plate reader software). Subsequently, the oxidative burst was initiated by the addition of PMA (final concentration 100 nM) and the cells incubated for 120 min at 37°C. Absorbance was then measured at 540 nm (isosbestic point of cytochrome c) and 550 nm (SpectraMax 340; Molecular Devices, Sunnyvale, CA, USA). Up for your consideration is a Molecular Devices SpectraMax 340 Microplate Spectrophotometer Model# 340 Condition is for parts or repair. 4. If depot service is the best option for you we will assist you with logistics to help you keep. ): 8. Read one sample or up to 384. 3 0. When reading optical density at wavelengths below 340 nm, special UVtransparent, disposable or quartz microplates and cuvettes that allow transmission of the far UV spectra must be used. After treatment, total cellular RNA was extracted with a cold RNA extraction solution (Ultraspec RNA;Call 1-800-745-2710. 2 GxP Software is the latest, most secure software to achieve full FDA 21 CFR Part 11 and EudraLex Annex 11 compliance with. 10× trypsin 0. Enzyme activities at high concentration were analyzed using a SpectraMAX 340 plate reader (Molecular Devices) at 380 nm in the kinetic mode at 37°C. The estimation of monodehydroascorbate reductase (MDHAR) (EC 1. The optical density (OD) at the wavelength of 450 nm was measured with a spectrometer (SpectraMax 340, Sunnyvale, CA, USA). These models can no longer be repaired. SpectraMax® Microplate Readers and SoftMax® Pro Software. Number of flashes. The reaction mixture consists of 100 mM potassium phosphate buffer (pH 7. 0112-0115G October2018 SpectraMax®M3 SpectraMax®M4 SpectraMax®M5 SpectraMax®M5e Multi-ModeMicroplateReaders UserGuideMolecular Devices SpectraMax Gemini XS Microplate Fluorometer $2,800. The antibodies originally defining this molecule by inducing apoptosis, anti-Fas and anti-APO-1 , were of the IgM or IgG3 subtype, respectively. gp70 blocking. Top and bottom reads are available for fluorescence, ti me-resolved fluorescence and luminescence detection. Photometric Range: 0. The SpectraMax® Plus 384 Microplate Reader from Molecular Devices can run both standard spectrophotometer and microplate reader applications on the same instrument. Absorbance values were read at 570 nm on an automated SpectraMAX 340 (Molecular Devices, Sunnyvale, CA, USA). This tunable visible absorbance reader is for 96- and 384-well microplates and acquires data in endpoint, kinetic, and spectral scan modes using wavelengths from 340-850 nm, tunable in 1. Manufacturer: Molecular Devices Model: SpectraMax The SpectraMax® 340 PC 384 Absorbance Microplate Reader from Molecular Devices provides the necessary tools for absorbance measurement in the visible range. Microplate Readers. Narrow bandwidths deliver increased measurement accuracy and linearity over a wide range of. The values were compared against a standard curve that was generated using known concentrations of IL6 to calculate concentration in the. The SpectraMax ABS readers use a grating monochromator to select the exact wavelength needed for every assay. Donor: 620 nm. ThermoMax performs automatic mixing before and between readings to ensure reliable and precise. 5 - 2. The growth. 00 USD. IMAP ® Technology from Molecular Devices enables rapid, homogeneous, and non-radioactive assay of kinases, phosphatases, and phosphodiesterases and is suited for both assay development and high-throughput screening. (7. The LDH level in the lavage fluid was calculated based on the standard curve, nor-malized with protein concentration and expressed as ng/mg protein. Our multi-mode plate readers provide great flexibility and include absorbance, fluorescence and luminescence with configurable options for fluorescence polarization (FP), time-resolved fluorescence (TRF), FRET, and AlphaScreen. ISM Equipment Classification (Group 1, Class A) This equipment is designated as. Methods: We investigated growth inhibition by varying concentrations of AMB in seven isolates of C. The activity was measured in a SpectraMAX 340 plate reader at 37°C and 410 nmol/l for 120 sec. Absorbance was measured at 450 nm by using a SpectraMax 340 microplate spectrophotometer (Molecular Device). Heme was preincubated for 5 min with A when added before the metals. Statistical analysis. TDP-43 stress granular cells were treated with 70% ice-cold. After the addition of sPLA2 (20 μ g), the reaction mixture was incubated for 40 min at 37°C, and the absorbance was read at 10 min intervals. SpectraMax 190 reads 96-well and strip-well plates. As discussed below, dilution of the culture was required because cell density is not proportional to A 600 when the values are above ;0. Molecular Devices ThermoMax is a high-efficiency, temperature-regulated, 96-well microplate reader. Top and bottom reads are available for fluorescence, ti me-resolved fluorescence and luminescence detection. The wavelength and scanning ranges are from 340–850 nm in 1 nm increments. Near bottom. Briefly, cells were seeded into a. The SpectraMax Vertical 1000 uses only the highest-efficacy ams-Osram LEDs with efficiencies up to 4. The absorbance at 540 nm was determined in a spectrophotometer (SPECTRAmax 340, Molecular Devices). MCE has not independently confirmed the accuracy of these methods. Donor filter: 616/10 nm. 2 M DEEMM were mixed in a microtube,. Briefly, cells were sequentially treated with terminal deoxynucleotidyl. 306 plate air cal fail,low light. 5. Although not statistically significant, pain was reduced by 2 NRS steps or more during supplemental treatment with naloxone in 36% of subjects when. VersaMax reads 96-well and strip-well plates. Immunofluorescence and Aggregation Analysis. Statistical AnalysisConsult Molecular Devices's entire SpectraMax i3x catalogue on MedicalExpo. Molecular Devices SpectraMax 384 Plus Microplate Reader Specifications. Acceptor: 665nm. SpectraMax M2, while top and bottom reads are possible on the SpectraMax M2 e. 5% phosphoric acid) in microplates at room temperature for 10 min. The results of ELISA optical density (OD) values in duplicates were averaged for the following analyses. SpectraMax 384 Plus. Not all manufacturers' microplates are the same with regard to design, materials, orIn a typical 100-μL assay with mixtures containing 50 mM MOPS, pH 6. Alves, Amelia K. Turn on the Spectramax 340PC spectrophotometer using the switch located next to the plug in the back on the right hand side (as you face the spec). Percent radical scavenging activity was determined by comparison with a DMSO containing control (shown in Table 3). The control panel consists of a 2-x-20-character LCD and eleven pressure-sensitive membrane keys that can be used to control some functions of the instrument. 0112-0126B 8 Chapter1:Introduction TheSpectraMax®340PC384,SpectraMax®190,andVersaMax™microplate. SoftMax Pro. The concentration of Alizarin Red S staining in the samples was determined by comparing the absorbance values with those. Created Date: 20051213090753Z measured using a SpectraMax 340 multiwell plate. Detection modalities include absorbance (UV-Vis Abs) and fluorescence intensity (FI) and have optical performance comparable to a top-of-the-line dedicated spectrofluorometer or. With SOFTmax PRO, the contents of the wells in a microplate can be mixed automatically by shaking before each read cycle, making it possible to. . CD154 detection in equine PBMC by an anti-human CD154 antibody (clone 5C8) was confirmed by Western blots and then applied for flow cytometry. Following cell culture under normoxic or hypoxic con-ditions, culture medium samples were obtained on completing the incubation period to measure atrial natriuretic peptide. The chamber contains. 315 can't find zero order. After then, 10% of ammonium hydroxide solution was added to neutralize the aqueous solution of the ARS extracts, and the absorbance values were measured using a SpectraMax® 340 plate reader at 405 nm. Details. When reading optical density at wavelengths below 340 nm, special UV-transparent, disposable or quartz microplates and cuvettes that allow transmission of the far UV spectra must be used. Top and bottom reads are available for fluorescence, time-resolved fluorescence and luminescence detection. Molecular Devices SpectraMax 340 PC 384 Microplate Reader. This is a used Molecular Devices SpectraMax 340PC Microplate Spectrophotometer. SpectraMax M2, while top and bottom reads are possible on the SpectraMax M2 e. 0. Description 1. Stat Fax 4700 Microstrip Reader. Plasma glucose levels were measured using FreeStyle Lite (Abbott Diabetes Care Ltd. CSF-1, IL-34 and calprotectin levels were analysed by ELISA according to instructions provided by the manufacturer (R&D Systems, Minneapolis, Minnesota, USA). The optical density of each well was determined immediately using a microplate reader (SpectraMax-340, Molecular Devices) set to 450 nm. 306 plate air cal. SpectraMax M2, while top and bottom reads are possible on the SpectraMax M2 e. SpectraMaxABS Plus,SpectraMaxM2,SpectraMaxM2e,SpectraMax M3,SpectraMaxM4,SpectraMaxM5,SpectraMaxM5e,SpectraMaxPlus 384 0200-7200 Multi-Mode ValidationPlate. HTRF-compatible SpectraMax Multi-Mode Readers. ac. Revvity. The contents of the wells in a microplate can be mixed automatically by shaking before each read cycle, which makes it possible to perform kinetic analysis of solid- Absorbance at 405 nm (Abs 405) was obtained for both plates before (background) and after 1-h incubation at 37°C using a microplate reader (SpectraMax 340 PC384, Molecular Devices). A microplate reader that can detect two or more applications is considered a multi-mode plate reader. addresses the needs of life science researchers who quantify very small amounts of DNA, RNA, oligos, and proteins. MCE has not independently confirmed the accuracy of these methods. For use with Spectramax 340 devices which have an 8-pin mini DIN serial socket. control. Optical density was measured at 570 nm using a microplate reader (SpectraMAX 340, Molecular Devices Co. edu Contact Phone: 216-368-8962. When reading at wavelengths below 340 nm, special UV-transparent, disposable or quartz microplates and cuvettes that allow transmission of the far UV spectra must be used. ). The light features a slim profile to prevent. View the Molecular Devices website. The Molecular Devices SpectraMAX absorbance microplate readers mimic a dual beam spectrophotometer with a patented multichannel optical design. After washing, the dye was eluted with 3% HCl and the absorbance measured at 665 nm with a SpectraMax 340 spectrophotometer (Molecular Devices, Sunnyvale, CA). The optical density value was measured at absorbance 450 nm by a microplate reader (SpectraMAX 340, molecular devices, USA) for cell viability. Molecular Devices Spectramax 340PC instructions. 6. ) at 490 nm. Top and bottom reads are available for fluorescence, ti me-resolved fluorescence and luminescence detection. Optical density was measured using a SpectraMAX 340 (Molecular Devices, Sunnyvale, CA). Wound healing assay. PGD2 concentrations in samples from treated cell cultures were determined using the linear range of a standard curve and are presented as percentages of the PGD2 levels measured in the solvent controls. For more sample throughput, the system canThe optical density (OD) at the wavelength of 450 nm was measured with a spectrometer (SpectraMax 340, Sunnyvale, CA, USA). Afterwards, absorbance at 595 nm was measured with spectrophotometer (SpectraMax 340, Molecular Devices, USA). The plate was covered and incubated for one hour at room temperature. FlexStation® 3 Multi-Mode Microplate Reader. 1: SpectraMax Plus 384 components. SOD activities were calculated from assays in which there was an inhibition of cytochrome c reduction of between 40 and 50%. The SpectraMax 340PC384 Microplate reader supports various absorbance assay categories, such as protein quantitation, endotoxin detection, microbial growth, ELISAs. 0112-0115J 9 Chapter1:Introduction TheSpectraMax®M3Multi-ModeMicroplateReader,SpectraMax®M4Multi-ModeMicroplate Reader,SpectraMax®M5Multi-ModeMicroplateReader,andSpectraMax®M5eMulti-ModeOpening the Drawer Manually on page The SpectraMax ABS can accommodate standard 96-well plates. 5), L-ascorbic acid (25. The inhibition percentage was determined by linear regression through ranges of 20 to 30. SpectraMax QuickDrop Micro-Volume Spectrophotometer. Product Keyword. Protein electrophoresis system (BioRad, Hercules, CA) and Odyssey Infrared Imaging System (Li-Cor Biosciences, Lincoln, NE) were used in western blot. The Catalina. The slope of the linear portion of the kinetic curve generated from each well was used to. BV-2 cells were treated with various concentrations of BT and/or LPS for 24 hFollowing incubation, absorbance was immediately measured at 405 nm with a SpectraMax 340 spectrophotometer (Molecular Devices, LLC. When reading optical density at wavelengths below 340 nm, special UV-transparent, disposable or quartz microplates and cuvettes that allow transmission of the far UV spectra must be used. The amount of liberated pNA product during incubations was determined using a standard curve obtained at 410 nm for various concentrations of a pNA stock solution in the SpectraMax 340 microplate reader. using SpectraMax i3 (Molecular devices, San Jose, USA). 024). 5. The SpectraMax ABS Plus reader was heated to 37ºC prior to setting up the. Background: Ketamine can act as a multifunctional neuroprotective agent by inhibiting oxidative stress, cellular dysfunction, and apoptosis. Background: A computerized machine, SPECTRAmax 340, was used to evaluate the recently reported phenomenon of antagonism of the polyene amphotericin B (AMB) in Candida albicans pre-exposed to the triazole fluconazole (FLZ). chen@case. Novak, Nathan J. The reaction mixture consists of 100 mM potassium phosphate buffer (pH 7. LAL Reagent Grade ™ Multi-well Plates (Lonza cat. SpectraMax Multi-Mode Microplate Readers. Changes in cell viability were reflected by changes in optical density detected at 450 nm, which was measured using a spectrophotometer microplate reader (SpectraMax 340; Molecular Devices, Sunnyvale, CA, USA). , Sunnyvale, CA). Media in plates were withdrawn by pipetting and 200 μl of DMSO was added to each well to solubilize the formazan crystals. Molecular Devices Spectramax 340 Absorbance Plate Reader (1997) Molecular Devices SpectraMax i3x Multi-Mode Microplate Reader (2016) Vernier UV-VIS Spectrophotometer (5) Vernier VIS Spectrophotometer (30) Infrared Spectroscopy. As a common indicator of equine T cell activation, cytokine induction. The SpectraMax ABS Plus, can accommodate standard 96-well plates and 384-well plates. -1 activity was determined MMP using an MMP-1 immunoassay kit (dY901; R&d Systems, Inc. When reading at wavelengths below 340 nm, special UV-transparent, disposable or quartz microplates allowing transmission of the deepThe SpectraMax i3x reader measures spectral-based absorbance, fluorescence, and luminescence with the added functionality of modular upgrades for western blot, imaging, and fast kinetics with injectors. Money Saved: $340. Percent viability was calculated by comparison with the control group. SpectraMax® Gemini™ EM Microplate Reader, Molecular Devices. The supernatants were mixed with an LDH assay reagent, which was purchased from Takara Bio Inc. 2. SPECTRAmax® 190. Labsystems Luminoskan RT Microplate Reader. 4. Spectrophotometer (SpectraMax 340). #25-340) Pyrogen-free Dilution Tubes (Lonza cat. Following incubation, 100 μl of solution was transferred to new wells and the color change in the solution was measured with a microtiter plate reader (SpectraMAX 340 Tunable Microplate Reader; Molecular Devices Ltd. Furthermore, the stain was extracted using 10% acetic acid, and its absorbance was measured at 595 nm in a microtiter plate reader (SpectraMax 340; Molecular Devices). Or scan up to 384 wells at any time to determine the best assay conditions. 5. Each experiment was performed in duplicate. Calculating % of total mediator release in RBL assay. Molecular Devices SpectraMax 340 PC 384 Microplate Reader. 2. Background: Ketamine can act as a multifunctional neuroprotective agent by inhibiting oxidative stress, cellular dysfunction, and apoptosis. One plate carrier adapter is provided with the instrument. 340 nm. These robust readers have been placed in labs from Antarctica to the. Subsequently, the oxidative burst was initiated by the addition of PMA (final concentration 100 nM) and the cells incubated for 120 min at 37°C. SpectraMax Multi-Mode Microplate Readers. A 340 μL of 50 mM borate buffer (pH 9), 240 μL methanol, 10 μL standard solution or sample, and 20 μL of 0. Subsequently, the oxidative burst was initiated by the addition of PMA (final concentration 100 nM) and the cells incubated for 120 min at 37°C. Wavelength Selection. 313 reference gain check fail. 340-850 nm: Wavelength Selection: Monochromator, turnable in 1 nm increments:. 8–250 nM) was measured as follows: the prothrombinase reaction proceeded for 3 min and was stopped by the addition of 10 mM EDTA. 6. Following HRP-conjugated secondary antibodies, goat anti-rat IgG (Kirkegaard & Perry. 30. 2 Chemical Reagents 1. SoftMax Pro. SpectraMax i3/i3x and Paradigm readers can be equipped with the HTRF detection cartridge. California Lightworks uses only the highest quality LEDs from Osram. 003 OD Stray light ≤ 0. The plates were read using an ELISA microplate reader (Molecular Devices SPECTRAMAX 340 PC 384, Sunnyvale, California, USA) at 450 nm. ScanLater Western Blot Detection Cartridge. After adding sample, the mixture was incubated for 30 min at 37°C, with one unit of enzymatic activity being defined as an increase in absorbance of 0. Our microplate readers and software are the industry’s most cited and have empowered life science researchers to advance protein and cell biology. SpectraMax Gemini EM & FLEXstation (bottom read) 1. 2 g/L solution; dilute 1:10 in HBSS. At that point halfway up the maximum transmittance value, the total width (nm) of the transmitted band is. Description. using SpectraMax i3 (Molecular devices, San Jose, USA). 00 USD. 1 0 100 80 60 40 20 0 4. SpectraMax 340PC 384 and SpectraMax Plus 384 read both 96-and 384-well microplates. The assay was carried out in a 96-well plate in a final volume of 100 µl containing 50 mmol/l Tris-HCl, pH 7. The absorbance was read at the wavelength of 570 nm using a microplate spectrophotometer (SpectraMax 340, Molecular Devices, CA, USA). Subsequently, the mixture was heated at 100 °C for 10. ATI Note: #1: The N-34-MLA is a replacement assembly which replaces an OEM lamp. Below is the list of Molecular Devices microplate readers and liquid handling instruments that are of out-of-production and are now considered obsolete. Analysis of ALA by Ehrlich’s Reagent Assay. Absorbance was then measured at 540 nm (isosbestic point of cytochrome c) and 550 nm (SpectraMax 340; Molecular Devices, Sunnyvale, CA, USA). When reading at wavelengths below 340 nm, special UV-transparent, disposable or quartz microplates and cuvettes that allow transmission of the far UV spectra must be used. 6) and 0. The relative cell viability was determined as the percentage of the absorbance value in the cells to the absorbance value of a control group. One plate carrier adapter is provided with the instrument. We selected the response at t=10 h for statistical analysis, because at that time point the response variation was largest. IC 50 values were determined by mathematical curve-fitting (SoftMaxPro Molecular Devices) and were defined as the drug concentration leading to 50% inhibition of net cell mass. Italic and boldface type are used for emphasis. 1% N-1-naphtylethylenediamine dihydrochloride, 2. Molecular Devices SpectraMax M5e Microplate Reader. 6) and 40 μL acetylacetone, then the mixture was heated at 100 °C for 10 min to. The values of IC 50 represent concentrations of compounds toA microplate reader (SpectraMax M5; Molecular Devices, Sunnyvale, CA) was used to measure the absorbance at 340 nm for the collected BPW (6) Stabilization protects islet integrity during respirometry in the Oroboros Oxygraph-2K analyzer Islets December 31, 2022 Justin J. ThermoMax performs automatic mixing before and between readings to ensure reliable and precise results. , Sunnyvale, CA, USA) at 595 nm. Introduction The SpectraMax® 340PC384 Reader from Molecular Devices provides everything needed to measure absorbance in the visible range, including temperature control, a robotics-compatible interface and SoftMax® Pro Data Acquisition and Analysis Software. Features showcased by this model include temperature control, a robotics-compatible interface and Data Analysis Software. , Fremont, CA, USA). 20. These lab workhorses are used in drug discovery, research, bioassay validation, and. Standard features include a cuvette port, spectral scanning in 1 nm increments, and up to six wavelengths per read. #ABS PLUS) SpectraMax® Plus 384 Microplate Reader (Molecular Devices cat. SpectraMax Microplate Readers. edu Contact Phone: 216-368-8962. Other technologies such as imaging. using a Molecular Dynamics Model SpectraMAX 340 PC microplate reader. Acceptor filter: 665/10 nm. Alkaline phosphatase (ALP) activity. 5. The absorbances were transformed to velocity of substrate consumption (nmol/min). Vessel Sprout Formation Assay. Samples containing 25 μg of protein were separated on 12. 5% (w/v) sodium dodecyl sulfate-polyacrylamide gels, and transferred to immobilon polyvinylidenedifluoride membranes (Millipore, Bedford, USA). Assay setup for a 384-well low-volume plate. Media in plates were withdrawn by pipetting and 200 μl of DMSO was added to each well to solubilize the formazan crystals. The SpectraMax® M Series Multi-Mode Microplate Readers are modular and upgradeable with a wide range of high performance capabilities ideal for life science research and drug discovery screening SoftMax® Pro 7. This assay measures the two major sugars in wine, glucose and fructose. After 72 h, 20 μl of 5 mg/ml MTT solution was added to each well and plates were incubated for 3 h at 37°C. ), which was proportional to the number of damaged cells. Absorbance at 405 nm (Abs 405) was obtained for both plates before (background) and after 1-h incubation at 37°C using a microplate reader (SpectraMax 340 PC384, Molecular Devices). SPECTRAmax 340PC Microplate Spectrophotometer Operator’s Manual 1-3 Introduction General Overview The SPECTRAmax ® 340PC incorporates a holographic grating. The cytotoxicity of the CDDP loaded CHAMs were done by treating the FaDu cells with DMEM supplemented with 0. #PLUS 384) MethodsSpectraMax 340 PC, Molecular Devices, USA was used as a 96-well microplate reader to test bioactivities. The absorbance is determined at 620 nm on a SpectraMax 340 PC 384. The SPECTRAmax 340PC can mea-The extracted stain was then transferred to a 96-well plate, and the absorbance at 562 nm was measured using a SpectraMax 340 plate reader/spectrophotometer(Molecular Devices Corp. Reagena Poc Puumala® was used for detection of IgM antibody against PUUV in NE patient sera at the Department of Clinical Virology,. SpectraMax i3x 和 SpectraMax iD5 微孔板读板机上使用 SoftMax Pro 软件中的预设方法进行测量(有关仪器特定设 置，请参见表 2 和表 3 )。在 SpectraMax i3x 和 SpectraMax iD5 读板机上进行了微孔板优化和读取高度调整，以确保最佳 的检测灵敏度和动态范围。 数据分析SpectraMax® Absorbance Microplate Readers can run both cuvette-based and microplate reader applications on the same instrument. The SpectraMax® ABS Plus Microplate Reader can run both cuvette-based and microplate reader applications on the same instrument. 00. The SpectraMax 384 Plus lets you get absorbance measurements from 190 to 1000 nm quickly for samples in test tubes, cuvettes, and 96- or 384-well microplates. ir Neuroprotection of a sesamin derivative, 1, 2-bis [(3-methoxy- phenyl) methyl] ethane-1, 2-dicaroxylic acid (MMEDA) againstEach SpectraMax® 340 PC 384 Microplate Reader has an optical system built around a monochromator. Includes: SpectraMax 340PC384. Molecular Devices SPECTRAmax. The initial rate of hydrolysis was determined using the early linear region of. Products : SpectraMax Plus 384 reader, SpectraMax 190 reader, SpectraMax 340PC 384 (Obsolete), SpectraMax 250 (Obsolete), SpectraMax 340 (Obsolete), VersaMax reader How does stray light affect my OD reading? The basic problem caused by stray light (light you don't want, getting into the read) is a falsely low OD seen. Nitric Oxide Assay.